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Validation and Evaluation of High-Resolution Orbitrap Mass Spectrometry on Molecular Characterization of Dissolved Organic Matter
journal contribution
posted on 2020-03-09, 11:42 authored by Qiong Pan, Xiaocun Zhuo, Chen He, Yahe Zhang, Quan ShiMolecular composition
of dissolved organic matter (DOM) is a hot
topic in subjects such as environmental science and geochemistry.
Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR
MS) has been applied to molecular composition characterization of
DOM successfully. However, high instrument and maintenance costs have
constrained its wider application. A high-resolution Orbitrap mass
spectrometer (Orbitrap MS) can provide approximately 500,000 resolving
power (at m/z 200), which is potentially
capable of characterizing the molecular composition of DOM. In this
paper, the application of high-resolution Orbitrap MS was evaluated
by comparing with FT-ICR MS in the aspect of resolution, mass distribution,
detection dynamic range, and isotopic peak intensity ratio. The impact
of instrument parameters of Orbitrap MS was further investigated,
which includes ionization, ion transfer, and mass detection. The result
shows that the high-resolution Orbitrap MS is capable or even preferable
for molecular characterization of DOM. However, the peak intensity
distributions are dependent on the instrument parameters, which could
affect the environmental impact assessment caused by the sample itself.
The result indicates that development of a universal and comparable
method is of great demand.