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Utilization of a Multiple Cloning Site as a Versatile Platform for DNA Triblock Copolymer Synthesis
journal contribution
posted on 2019-10-04, 14:34 authored by Jeehae Shin, Sheng LiDNA-containing
block copolymers have utility in a wide range of
biomedical applications. However, synthesis of these hybrid materials,
especially ones with complex chain structures, remains to be a major
challenge. Here, we report the use of a combination of restriction
enzyme sites and ligation enzymes to synthesize DNA triblock copolymers.
In contrast to triblock structures held together by DNA hybridization,
the newly synthesized DNA triblocks have all blocks connected by covalent
bonds. The improved stability of the triblocks against environmental
factors such as urea denaturing is confirmed. Furthermore, we incorporate
a multiple cloning site (MCS) into the DNA block copolymers and show
that the restriction sites can be cut by their corresponding restriction
enzymes, generating diblocks with different sticky ends. By utilizing
these sticky ends of specific sequences, the cut diblocks are further
ligated to create a variety of triblock copolymers with different
DNA center blocks and synthetic polymer end blocks. This study presents
a versatile platform based on MCS for the synthesis and regeneration
of a range of DNA-containing block copolymers.
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DNA hybridizationVersatile PlatformDNA Triblock Copolymer Synthesis DNA-containing block copolymerstriblock structurespolymer end blocksDNA-containing block copolymersDNA triblock copolymersDNA block copolymersrestriction enzymescovalent bondstriblock copolymersDNA center blocksrestriction enzyme sitesurea denaturingligation enzymesDNA triblocksrestriction sitesMultiple Cloning Sitechain structuresMCS
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