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Unusual Topological RNA Architecture with an Eight-Stranded Helical Fragment Containing A‑, G‑, and U‑Tetrads
journal contribution
posted on 2017-02-08, 00:00 authored by Chao-Da Xiao, Takumi Ishizuka, Xiao-Qing Zhu, Yue Li, Hiroshi Sugiyama, Yan XuHuman
telomeric RNA performs various cellular functions such as
telomere length regulation, heterochromatin formation, and chromosome
end protection. Using a combination of nuclear magnetic resonance,
circular dichroism, and gel electrophoresis, we observed an unusual
topological structure formed by human telomere RNA r(GUUAGGGU). Our
results showed that every set of four strands formed a parallel G-quadruplex
as symmetry-related units containing four G-tetrads, two U-tetrads,
and one A-tetrad. An eight-stranded helical fragment containing A-,
G-, and U-tetrads provided a central intercalated scaffold that connected
two G-quadruplex units in an alternating antiparallel arrangement,
giving rise to a novel RNA architecture. This higher order RNA structure
is so stable that it would be surprising if similar structures do
not occur in nature. Our findings provide a new insight into the behavior
of human telomeric RNA molecules.
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chromosome end protectiontelomere length regulationtopological structuregel electrophoresisG-quadruplex unitseight-stranded helical fragmentheterochromatin formationsymmetry-related unitsEight-Stranded Helical Fragment ContainingRNA moleculesorder RNA structureantiparallel arrangementnovel RNA architectureintercalated scaffoldUnusual Topological RNA Architecture
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