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Unique Sensing Interface That Allows the Development of an Electrochemical Immunosensor for the Detection of Tumor Necrosis Factor α in Whole Blood
journal contribution
posted on 2016-11-24, 00:00 authored by Cheng Jiang, Muhammad Tanzirul Alam, Saimon Moraes Silva, Safura Taufik, Sanjun Fan, J. Justin GoodingElectrochemical affinity
biosensors that can operate in whole blood
are a rarity because biofouling of electrode surfaces compromises
the performance of the final device. The common anti-biofouling layers
that can be applied to electrodes, poly(ethylene glycol) (PEG) or
oligo(ethylene glycol) (OEG) layers, form a high impedance layer on
the electrode, effectively passivating the electrode. In response
to this issue, we have developed effective anti-biofouling chemistry,
that employs short chain zwitterionic species, derived from aryl diazonium
salts, that give low impedance layers compatible with amperometry.
Herein, we demonstrate the application of this surface chemistry to
mixed layers of phenyl phosphorylcholine (PPC) and phenyl butyric
acid (PBA), to develop immunosensors that can be used in whole blood.
The capability of these new modification layers is demonstrated with
an immunosensor for detecting tumor necrosis factor α in whole
blood. The immunosensor is shown to specifically and precisely detect
TNF-α in whole blood samples with a minimum detection limit
of 10 pg/mL with a wide linear range of 0.01 ng/mL to 500 ng/mL. The
results are comparable with those from commercial ELISA kit, indicating
the developed immunosensor has great potential for future clinic use.
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Tumor Necrosis Factor αdetection limitphenyl phosphorylcholineelectrode surfaces compromisessurface chemistryfuture clinic useWhole Blood Electrochemical affinity biosensorsanti-biofouling layersanti-biofouling chemistrychain zwitterionic speciesimpedance layerPBAmodification layersblood samplesaryl diazonium saltsPEGimpedance layersTNF -αOEGElectrochemical ImmunosensorELISA kitphenyl butyric acidPPCimmunosensortumor necrosis factor α
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