posted on 2019-11-11, 23:43authored byJuan Yue, Li Li, Lei Cao, Minghui Zan, Dian Yang, Zheng Wang, Zhimin Chang, Qian Mei, Peng Miao, Wen-Fei Dong
It
is well known that the calcium ion is essential for maintaining
life activities in living organisms, and it is of great significance
to detect the intracellular calcium concentration. For the detection
of calcium ions, we developed a new type of fluorescent carbon dots
(CDs), whose surface was modified by ethylenebis(oxyethylenenitrilo)tetraacetic
acid (EGTA) through a secondary hydrothermal method. This is a simple
and convenient chemical preparation method because all reactions are
carried out in the same autoclave, and the final product is directly
the EGTA-modified CDs. The CDs exhibit bright blue fluorescence, and
as the calcium concentration increases, the fluorescence intensity
drops sharply. The fluorescence quenching correlates with the concentration
of calcium ions and has a good linearity in the range of 15–300
μM with a detection limit of 0.38 μM. The experimental
results confirmed that the detection of calcium ions by CDs is a static
fluorescence quenching process. Also, cytotoxicity test and cellular
imaging experiments have shown that the CDs are nontoxic and biocompatible.