ac9b00607_si_001.pdf (2.32 MB)
Two-Photon Probes for Golgi Apparatus: Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy
journal contribution
posted on 2019-03-28, 00:00 authored by Ji-Woo Choi, Seung Taek Hong, Mun Seok Kim, Kyu Cheol Paik, Man So Han, Bong Rae ChoWe
have developed blue- and yellow-emitting two-photon probes (BGolgi-blue
and PGolgi-yellow) from 6-(benzo[d]oxazol-2-yl)-2-naphthalylamine
and 2,5-bis(benzo[d]oxazol-2-yl)pyrazine derivatives
as the fluorophores and trans-Golgi-network peptide (SDYQRL) as the
Golgi-apparatus-targeting moiety. HeLa cells labeled with BGolgi-blue
and PGolgi-yellow emitted two-photon-excited fluorescence at 462 and
560 nm, respectively, with effective two-photon-action cross-section
values of 1860 and 1600 × 10–50 cm4·s/photon, respectively. The probes can detect the Golgi apparatus
in live cells and deep inside live tissue via two-photon microscopy
at widely separated wavelength regions with high selectivity and minimal
pH interference, and they are photostable and have low cytotoxicity.