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Translocation of an Intracellular Protein via Peptide-Directed Ligation
journal contribution
posted on 2016-12-21, 00:00 authored by Christiane Stiller, Dennis M. Krüger, Nicolas Brauckhoff, Marcel Schmidt, Petra Janning, Hazem Salamon, Tom N. GrossmannLigand-directed reactions
allow chemical transformations at very
low reactant concentrations and can thus provide access to efficient
approaches for the post-translational modification of proteins. The
development of these proximity-induced reactions is hampered by the
number of appropriate ligands and the lack of design principles. Addressing
these limitations, we report a proximity-induced labeling system which
applies a moderate affinity peptide ligand. The design process was
structure-guided and supported by molecular dynamics simulations.
We show that selective protein labeling can be performed inside living
cells enabling the subcellular translocation of a protein via ligand-directed
chemistry for the first time.
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proximity-induced reactionschemical transformationsproteindesign processIntracellular Proteinsubcellular translocationdesign principlesligand-directed chemistrydynamics simulationsreactant concentrationspost-translational modificationPeptide-Directed Ligation Ligand-directed reactionsaffinity peptide ligand
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