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Transglycosylation of Naringin by Bacillus stearothermophilus Maltogenic Amylase To Give Glycosylated Naringin
journal contribution
posted on 1999-08-14, 00:00 authored by So Jin Lee, Jae-Cherl Kim, Myo Jeong Kim, Motomitsu Kitaoka, Cheon Seok Park, Su Yong Lee, Moon-Jin Ra, Tae Wha Moon, John F. Robyt, Kwan Hwa ParkNaringin, a bitter compound in citrus fruits, was transglycosylated by Bacillus stearothermophilus
maltogenic amylase reaction with maltotriose to give a series of mono-, di-, and triglycosylnaringins.
Glycosylation products of naringin were observed by TLC and HPLC. The major glycosylation product
was purified by using a Sephadex LH-20 column. The sturcture was determined by using MALDI-TOF MS, methylation analysis, and 1H and 13C NMR. The major transglycosylation product was
maltosylnaringin, in which the maltose unit was attached by an α-1→6 glycosidic linkage to the
d-glucose moiety of naringin. This product was 250 times more soluble in water and 10 times less
bitter than naringin.
Keywords: Naringin; maltogenic amylase from Bacillus stearothermophilus; maltosylnaringin;
transglycosylation
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Bacillus stearothermophilusmethylation analysistransglycosylation productnaringin13 C NMR250 timesglycosylation productGlycosylation productsLHHPLCmaltosylnaringinTLC10 times1 HGlycosylated Naringin NaringinBacillus stearothermophilus Maltogenic AmylaseBacillus stearothermophilus maltogenic amylase reactionmaltose unitMSmaltogenic amylase
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