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Toward Upscaled Biocatalytic Preparation of Lactone Building Blocks for Polymer Applications
journal contribution
posted on 2018-06-12, 00:00 authored by Marie
A. F. Delgove, Matthew T. Elford, Katrien V. Bernaerts, Stefaan M. A. De WildemanAlthough
Baeyer–Villiger monooxygenases (BVMOs) have gained
attention in recent years, there are few cases of their upscaled application
for lactone synthesis. A thermostable cyclohexanone monooxygenase
from Thermocrispum municipale (TmCHMO)
was applied to the oxidation of 3,3,5-trimethylcyclohexanone using
a glucose dehydrogenase (GDH) for cofactor regeneration. The reaction
progress was improved by optimizing the biocatalyst loading, with
investigation into oxygen limitations. The product concentration and
productivity were increased by keeping the substrate concentration
below the inhibitory level via continuous substrate feeding (CSF).
This substrate feeding strategy was evaluated against two biphasic
reactions using either toluene or n-butyl acetate
as immiscible organic solvents. A product concentration of 38 g L–1 and a space-time yield of 1.35 g L–1 h–1 were achieved during the gram-scale synthesis
of the two regioisomeric lactones by applying the CSF strategy. These
improvements contribute to the large-scale application of BVMOs in
the synthesis of branched building blocks for polymer applications.