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Thiamine Pyrophosphate Stimulates Acetone Activation by Desulfococcus biacutus As Monitored by a Fluorogenic ATP Analogue
journal contribution
posted on 2014-06-20, 00:00 authored by Olga B. Gutiérrez Acosta, Norman Hardt, Stephan M. Hacker, Tobias Strittmatter, Bernhard Schink, Andreas MarxAcetone can be degraded by aerobic
and anaerobic microorganisms.
Studies with the strictly anaerobic sulfate-reducing bacterium Desulfococcus biacutus indicate that acetone degradation
by these bacteria starts with an ATP-dependent carbonylation reaction
leading to acetoacetaldehyde as the first reaction product. The reaction
represents the second example of a carbonylation reaction in the biochemistry
of strictly anaerobic bacteria, but the exact mechanism and dependence
on cofactors are still unclear. Here, we use a novel fluorogenic ATP
analogue to investigate its mechanism. We find that thiamine pyrophosphate
is a cofactor of this ATP-dependent reaction. The products of ATP
cleavage are AMP and pyrophosphate, providing first insights into
the reaction mechanism by indicating that the reaction proceeds without
intermediate formation of acetone enol phosphate.