pr500833v_si_001.xlsx (4.66 MB)
The Impact of High-Fat Diet on Metabolism and Immune Defense in Small Intestine Mucosa
dataset
posted on 2015-12-17, 06:43 authored by Jacek R. Wiśniewski, Alexandra Friedrich, Thorsten Keller, Matthias Mann, Hermann KoepsellImproved
procedures for sample preparation and proteomic data analysis
allowed us to identify 7700 different proteins in mouse small intestinal
mucosa and calculate the concentrations of >5000 proteins. We compared
protein concentrations of small intestinal mucosa from mice that were
fed for two months with normal diet (ND) containing 34.4% carbohydrates,
19.6% protein, and 3.3% fat or high-fat diet (HFD) containing 25.3%
carbohydrates, 24.1% protein, and 34.6% fat. Eleven percent of the
quantified proteins were significantly different between ND and HFD.
After HFD, we observed an elevation of proteins involved in protein
synthesis, protein N-glycosylation, and vesicle trafficking.
Proteins engaged in fatty acid absorption, fatty acid β-oxidation,
and steroid metabolism were also increased. Enzymes of glycolysis
and pentose phosphate cycle were decreased, whereas proteins of the
respiratory chain and of ATP synthase were increased. The protein
concentrations of various nutrient transporters located in the enterocyte
plasma membrane including the Na+-d-glucose cotransporter
SGLT1, the passive glucose transporter GLUT2, and the H+-peptide cotransporter PEPT1 were decreased. The concentration of
the Na+,K+-ATPase, which turned out to be the
most strongly expressed enterocyte transporter, was also decreased.
HFD also induced concentration changes of drug transporters and of
enzymes involved in drug metabolism, which suggests effects of HFD
on pharmacokinetics and toxicities. Finally, we observed down-regulation
of antibody subunits and of components of the major histocompatibility
complex II that may reflect impaired immune defense and immune tolerance
in HFD. Our work shows dramatic changes in functional proteins of
small intestine mucosa upon excessive fat consumption.