Systematic Investigation of Molecular Recognition Ability in FET-Based Chemical Sensors Functionalized with a Mixed Self-Assembled Monolayer System

Exploring new strategies for simple and on-demand methods of manipulating the sensing ability of sensor devices functionalized with artificial receptors embedded in a molecular assembly is important to realizing high-throughput on-site sensing systems based on integrated and miniaturized devices such as field-effect transistors (FETs). Although FET-based chemical sensors can be used for rapid, quantitative, and simultaneous determination of various desired analytes, detectable targets in conventional FET sensors are currently restricted owing to the complicated processes used to prepare sensing materials. In this study, we investigated the relationship between the sensing features of FETs and the nanostructures of mixed self-assembled monolayers (mSAMs) for the detection of biomolecules. The FET devices were systematically functionalized using mixtures of benzenethiol derivatives (4-mercaptobenzoic acid and benzenethiol), which changed the nanostructure of the SAMs formed on gold sensing electrodes. The obtained cross-reactivity in the FETs modified with the mSAMs was derived from the multidimensional variations of the SAM characteristics. Our successful demonstration of continuous control of the molecular recognition ability in the FETs by applying the mSAM system could lead to the development of next-generation versatile analyzers, including chemical sensor arrays for the determination of multiple analytes anytime, anywhere.