am201795g_si_001.pdf (244.41 kB)
Surface Modification of Smooth Poly(l-lactic acid) Films for Gelatin Immobilization
journal contribution
posted on 2012-02-22, 00:00 authored by Hai Li, Yun Xia, Jumiati Wu, Qiyuan He, Xiaozhu Zhou, Gang Lu, Lei Shang, Freddy Boey, Subbu S. Venkatraman, Hua ZhangPoly(l-lactic acid) (PLLA) is widely used in
drug delivery
and medical implants. Surface modification of PLLA with functional
groups to immobilize gelatin or other extracellular matrix proteins
is commonly used to improve its cellular affinity. In this work, we
use the oxygen plasma to treat PLLA film followed by modification
with
organosilanes with different functional groups, such
as amine, epoxy, and aldehyde groups. Gelatin is then immobilized
on the modified PLLA film, which is confirmed by water contact angle
measurement, atomic force microscopy (AFM), and laser scanning confocal
microscopy (LSCM). Among the used organosilanes, aminosilane is the
best one for modification of PLLA used for immobilization of gelatin
with the highest efficiency. Moreover, the cellular affinity of gelatin-immobilized
PLLA is studied through the evaluation of cell proliferation and focal
adhesion using the human umbilical vein endothelial cells (HUVECs).
Our experimental results show that the gelatin immobilized on aminosilane-
and aldehyde-silane-modified PLLA improves the cellular affinity of
HUVECs, whereas that immobilized on epoxy-silane-modified PLLA does
not show significant improvement on the cell proliferation.