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Surface Effects of Ultrasmall Iron Oxide Nanoparticles on Cellular Uptake, Proliferation, and Multipotency of Neural Stem Cells
journal contribution
posted on 2020-01-17, 21:43 authored by Seungjo Park, Jennifer A. Sherwood, Rebecca M. Hauser, Angelo J. Antone, Brianna T. Beswick, Farah D. Lubin, Yuping Bao, Yonghyun KimUltrasmall iron oxide
nanoparticles (USIONPs) have been recently
developed as labeling probes for T2 magnetic
resonance imaging contrast agents. However, their use in stem cell
tracking has been limited, especially as T1 contrast agents. In this study, we studied the effects of USIONP
surface coatings on proliferation, cellular uptake, and multipontency
of established and primary neural stem cells (NSCs). USIONPs were
functionalized with gluconic acid (GA), tannic acid (TA), and hyaluronic
acid (HA) to label the NSCs. All functionalized USIONPs were characterized
as T1 contrast agents via relaxivity measurements.
Direct functionalization with TA and HA coating promoted NSC proliferation
and enhanced cellular uptake in a dose-dependent manner compared to
those with GA. Furthermore, HA coating showed enhanced cell proliferation
and cellular uptake in primary NSCs depending on the HA molecular
weight. Stem cell characteristics were well-maintained, verified by
neurosphere formation and gene expression of stemness and differentiation
markers. Collectively, we demonstrated that NSC proliferation, cellular
uptake, and multipotency can be enhanced using various surface coating
strategies of USIONPs.