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Surface-Derivatized Nanoceria with Human Carbonic Anhydrase II Inhibitors and Fluorophores: A Potential Drug Delivery Device
journal contribution
posted on 2007-06-21, 00:00 authored by Swanand Patil, Serge Reshetnikov, Manas K. Haldar, Sudipta Seal, Sanku MallikHuman carbonic anhydrase (hCAII) is a metalloenzyme that catalyzes the reversible hydration of carbon
dioxide to bicarbonate and is associated with glaucoma (a major cause of blindness). The present study focuses
on the use of cerium oxide nanoparticles (nanoceria) as a potential delivery device for hCAII inhibitors.
Carboxybenzenesulfonamide, an inhibitor of the hCAII enzyme, was attached to nanoceria particles using
epichlorohydrin as an intermediate linkage. Along with the CA inhibitor, a fluorophore (carboxyfluorescein)
was also attached on the nanoparticles to enable the tracking of the nanoparticles in vitro as well as in vivo.
X-ray photoelectron spectroscopic studies carried out at each reaction step confirmed the successful
derivatization of the nanoceria particles. The attachment of carboxyfluorescein was also confirmed by confocal
fluorescence microscopy. Preliminary studies suggest that carboxybenzenesulfonamide-functionalized nanoceria
retains its inhibitory potency for hCAII.