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Supramolecular Encapsulation of Small-Ultrared Fluorescent Proteins in Virus-Like Nanoparticles for Noninvasive In Vivo Imaging Agents
journal contribution
posted on 2020-05-07, 19:06 authored by Fabian
C. Herbert, Olivia R. Brohlin, Tyler Galbraith, Candace Benjamin, Cesar A. Reyes, Michael A. Luzuriaga, Arezoo Shahrivarkevishahi, Jeremiah J. GassensmithIcosahedral
virus-like particles (VLPs) derived from bacteriophages
Qβ and PP7 encapsulating small-ultrared fluorescent protein
(smURFP) were produced using a versatile supramolecular capsid disassemble–reassemble
approach. The generated fluorescent VLPs display identical structural
properties to their nonfluorescent analogs. Encapsulated smURFP shows
indistinguishable photochemical properties to its unencapsulated counterpart,
exhibits outstanding stability toward pH, and produces bright in vitro
images following phagocytosis by macrophages. In vivo imaging allows
the biodistribution to be imaged at different time points. Ex vivo
imaging of intravenously administered encapsulated smURFP reveals
a localization in the liver and kidneys after 2 h blood circulation
and substantial elimination after 16 h of imaging, highlighting the
potential application of these constructs as noninvasive in vivo imaging
agents.
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time pointsnonfluorescent analogsEncapsulated smURFPsupramolecular capsid16 h2 h blood circulationVirus-Like NanoparticlesEx vivo imagingPP 7 encapsulating small-ultraredvivo imagingvivo imaging agentsunencapsulated counterpartSupramolecular EncapsulationVivo Imaging Agents Icosahedral virus-like particlesbacteriophages Q βVLPs displayencapsulated smURFP
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