jf950396b_si_001.pdf (210.87 kB)
Structure Elucidation of Lolitrem F, a Naturally Occurring Stereoisomer of the Tremorgenic Mycotoxin Lolitrem B, Isolated from Lolium perenne Infected with Acremonium lolii
journal contribution
posted on 1996-09-18, 00:00 authored by Sarah C. Munday-Finch, Alistair L. Wilkins, Christopher O. Miles, Richard M. Ede, Ralph A. ThomsonA new lolitrem, lolitrem F (2), was isolated from
endophyte-infected perennial ryegrass. Its structure
was shown by mass spectrometry and one- and two-dimensional NMR
spectroscopy to be a 31,35-cis-fused isomer of lolitrem B. Base-catalyzed
epimerization of 2 and lolitrem B (1) afforded
their
31-epimers (4 and 3, respectively).
Comparison with spectral data for 1 and
31-epi-lolitrem B (3)
established lolitrem F to be 35-epi-lolitrem B.
Compounds 1, 2, and 4 were
equally tremorgenic in
a mouse bioassay, but 3 was nontremorgenic.
Base-catalyzed exchange of H-31 was found to permit
efficient incorporation of deuterium (and potentially, therefore, of
tritium) into 1 and 3.
Keywords: Acremonium lolii; Lolium perenne;
endophyte; lolitrem; tremor; ryegrass staggers;
neurotoxin; mycotoxin