jf5b04824_si_001.pdf (1.42 MB)
Stage-Specific Fatty Acid Fluxes Play a Regulatory Role in Glycerolipid Metabolism during Seed Development in Jatropha curcas L.
journal contribution
posted on 2015-12-23, 00:00 authored by Bharatula
Sri Krishna Chaitanya, Sumit Kumar, Shiva Shanker Kaki, Marrapu Balakrishna, Mallampalli Sri Lakshmi Karuna, Rachapudi
Badari Narayana Prasad, Pidaparty Seshadri Sastry, Attipalli Ramachandra ReddyThe present study describes the changes
in lipid profile as well
as fatty acid fluxes during seed development in Jatropha
curcas L. Endosperm from 34, 37, and 40 days after
anthesis (DAA), incubated with [14C]acetate, showed significant
synthesis of phosphatidylcholine (PC) at seed maturation. The fatty
acid methyl ester profile showed PC from 34 DAA was rich in palmitic
acid (16:0), whereas PC from 37 and 40 DAA was rich in oleic acid
(18:1n-9). Molecular species analysis of diacylglycerol (DAG) indicated
DAG (16:0/18:2n-6) was in abundance at 34 DAA, whereas DAG (18:1n-9/18:2n-6)
was significantly high at 40 DAA. Triacylglycerol (TAG) analysis revealed
TAG (16:0/18:2n-6/16:0) was abundant at 34 DAA, whereas TAG (18:1n-9/18:2n-6/18:1n-9)
formed the majority at 40 DAA. Expression of two types of diacylglycerol
acyltransferases varied with seed maturation. These data demonstrate
stage-specific distinct pools of PC and DAG synthesis during storage
TAG accumulation in Jatropha seed.