ab8b00393_si_001.pdf (576.53 kB)
Spiral Layer-by-Layer Micro-Nanostructured Scaffolds for Bone Tissue Engineering
journal contribution
posted on 2018-04-25, 00:00 authored by Ohan S. Manoukian, Aja Aravamudhan, Paul Lee, Michael R. Arul, Xiaojun Yu, Swetha Rudraiah, Sangamesh G. KumbarThis
Article reports the fabrication and characterization of composite
micro-nanostructured spiral scaffolds functionalized with nanofibers
and hydroxyapatite (HA) for bone regeneration. The spiral poly(lactic
acid-co-glycolic acid) (PLGA) porous microstructure
was coated with sparsely spaced PLGA nanofibers and HA to enhance
surface area and bioactivity. Polyelectrolyte-based HA coating in
a layer-by-layer (LBL) fashion allowed 10–70 μM Ca2+/mm2 incorporation. These scaffolds provided a
controlled release of Ca2+ ions up to 60 days with varied
release kinetics accounting up to 10–50 μg. Spiral scaffolds
supported superior adhesion, proliferation, and osteogenic differentiation
of rat bone marrow stromal cells (MSCs) as compared to controls microstructures.
Spiral micro-nanostructures supported homogeneous tissue ingrowth
and resulted in bone-island formation in the center of the scaffold
as early as 3 weeks in a rabbit ulnar bone defect model. In contrast,
control cylindrical scaffolds showed tissue ingrowth only at the surface
because of limitations in scaffold transport features.