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Spatially Resolved Membrane Transport in a Single Cell Imaged by Second Harmonic Light Scattering
journal contribution
posted on 2019-03-26, 00:00 authored by Mohammad Sharifian Gh., Michael J. Wilhelm, Michael Moore, Hai-Lung DaiWe
demonstrate that time-resolved second harmonic (SH) light scattering,
when applied as an imaging modality, can be used to spatially resolve
the adsorption and transport rates of molecules diffusing across the
membrane in a living cell. As a representative example, we measure
the passive transport of the amphiphilic ion, malachite green, across
the plasma membrane in living human dermal fibroblast cells. Analysis
of the time-resolved SH images reveals that membrane regions, which
appear to be enduring higher stress, exhibit slower transport rates.
It is proposed that this stress-transport relation may be a result
of local enrichment of membrane rigidifiers as part of a response
to maintain membrane integrity under strain.