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Soybean Mutants Lacking Abundant Seed Storage Proteins Are Impaired in Mobilization of Storage Reserves and Germination
journal contribution
posted on 2020-03-31, 13:39 authored by Xiaoshuang Wei, Won-Seok Kim, Bo Song, Nathan W. Oehrle, Shanshan Liu, Hari B. KrishnanSpontaneous and radiation-induced
mutants of soybean, despite loss
of abundant seed proteins, have been reported to grow and reproduce
normally without any apparent physiological abnormalities. Here, we
report the development and characterization of a soybean line (BSH-2)
that lacks several abundant seed storage proteins. One-dimensional
and high-resolution two-dimensional gel electrophoresis revealed the
absence of the α′ and α subunits of β-conglycinin
and G1, G2, G4, and G5 glycinin in the newly developed mutant line
(BSH-2). Like our earlier developed soybean mutant line (BSH-3), the
seeds of BSH-2 also accumulated high levels of free amino acids as
compared with wild-type DN47 seeds. An examination of the germination
rates revealed that both BSH-2 and BSH-3 had significantly lower germination
rates compared with the parent line DN47. Two-dimensional gel electrophoresis
analysis demonstrated that these mutants had slower rates of mobilization
of seed storage proteins. The delayed mobilization of storage proteins
in BSH-2 and BSH-3 seeds was also correlated with a delayed induction
of proteolytic activity in the mutants when compared to DN47. Similarly,
qRT-PCR analysis revealed distinct expression pattern of genes involved
in proteolytic pathway in the mutants when compared to DN47. Transmission
electron microscopy observation of soybean seeds at two germination
stages revealed striking differences in the breakdown of protein storage
vacuoles and lipid bodies in the mutants. Our study demonstrates that
BSH-2 and BSH-3 are compromised in mobilization of storage reserves
and the absence of abundant storage proteins may affect the seed germination
efficiency and post-germinative seedling establishment.
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germination ratesG 5 glycininBSH -3 seedsDN 47. Transmission electron microscopy observationparent line DN 47. Two-dimensional gel electrophoresis analysisseed germination efficiencywild-type DN 47 seedsAbundant Seed Storage Proteinsprotein storage vacuolesmutantpost-germinative seedling establishmentseed storage proteinsBSH -2BSH -3storage proteins
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