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Solvent Stability Study with Thermodynamic Analysis and Superior Biocatalytic Activity of Burkholderia cepacia Lipase Immobilized on Biocompatible Hybrid Matrix of Poly(vinyl alcohol) and Hypromellose
journal contribution
posted on 2014-12-26, 00:00 authored by Kirtikumar
C. Badgujar, Bhalchandra M. BhanageIn the present study, we have synthesized
a biocompatible hybrid
carrier of hypromellose (HY) and poly(vinyl alcohol) (PVA) for immobilization
of Burkholderia cepacia lipase (BCL).
The immobilized biocatalyst HY:PVA:BCL was subjected to determination
of half-life time (τ) and deactivation rate constant (KD) in various organic solvents. Biocatalyst
showed higher τ-value in a nonpolar solvent like cyclohexane
(822 h) as compared to that of a polar solvent such as acetone (347
h), which signifies better compatibility of biocatalyst in the nonpolar
solvents. Furthermore, the KD-value was found to be less in cyclohexane (0.843 × 10–3) as compared to acetone (1.997 × 10–3), indicating
better stability in the nonpolar solvents. Immobilized-BCL (35 mg)
was sufficient to achieve 99% conversion of phenethyl butyrate (natural
constituent of essential oils and has wide industrial applications)
using phenethyl alcohol (2 mmol) and vinyl butyrate (6 mmol) at 44
°C in 3 h. The activation energy (Ea) was found to be lower for immobilized-BCL than crude-BCL, indicating
better catalytic efficiency of immobilized lipase BCL. The immobilized-BCL
reported 6-fold superior biocatalytic activity and 8 times recyclability
as compared to crude-BCL. Improved catalytic activity of immobilized
enzyme in nonpolar media was also supported by thermodynamic activation
parameters such as enthalpy (ΔH⧧), entropy (ΔS⧧) and Gibb’s
free energy (ΔG⧧) study,
which showed that phenethyl butyrate synthesis catalyzed by immobilized-BCL
was feasible as compared to crude-BCL. The present work explains a
thermodynamic investigation and superior biocatalytic activity for
phenethyl butyrate synthesis using biocompatible immobilized HY:PVA:BCL
in nonaqueous media for the first time.
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Keywords
biocatalytic activityphenethyl butyrate synthesisactivationacetoneSuperior Biocatalytic ActivityHYbiocatalystBurkholderia cepacia Lipase ImmobilizedSolvent Stability Study8 times recyclabilityBiocompatible Hybrid MatrixBCLBurkholderia cepacia lipasemmolPVAKDnonpolar solventsmediacyclohexanebiocompatible
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