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Solid-State Nanopore Easy Chip Integration in a Cheap and Reusable Microfluidic Device for Ion Transport and Polymer Conformation Sensing
journal contribution
posted on 2018-10-04, 00:00 authored by Jean Roman, Olivier Français, Nathalie Jarroux, Gilles Patriarche, Juan Pelta, Laurent Bacri, Bruno Le PioufleSolid-state
nanopores have a huge potential in upcoming societal
challenging applications in biotechnologies, environment, health,
and energy. Nowadays, these sensors are often used within bulky fluidic
devices that can cause cross-contaminations and risky nanopore chips
manipulations, leading to a short experimental lifetime. We describe
the easy, fast, and cheap innovative 3D-printer-helped protocol to
manufacture a microfluidic device permitting the reversible integration
of a silicon based chip containing a single nanopore. We show the
relevance of the shape of the obtained channels thanks to finite elements
simulations. We use this device to thoroughly investigate the ionic
transport through the solid-state nanopore as a function of applied
voltage, salt nature, and concentration. Furthermore, its reliability
is proved through the characterization of a polymer-based model of
protein–urea interactions on the nanometric scale thanks to
a hairy nanopore.
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Solid-state nanopores3 D-printer-helped protocolpolymer-based modelmicrofluidic deviceSolid-State Nanopore Easy Chip IntegrationReusable Microfluidic DeviceIon Transportfluidic devicesnanometric scale thankschannels thanksPolymer Conformationsalt natureelements simulationsnanopore chips manipulations
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