cr9b00379_si_001.pdf (137.03 kB)
Small Molecule as Fluorescent Probes for Monitoring Intracellular Enzymatic Transformations
journal contribution
posted on 2019-11-13, 22:13 authored by Harwinder Singh, Karishma Tiwari, Rajeshwari Tiwari, Sumit Kumar Pramanik, Amitava DasAll cellular processes
are the results of synchronized actions
of several intracellular biochemical pathways. Recent emphasis is
to visualize such pathways using appropriate small molecular reagents,
dye-labeled proteins, and genetically encoded fluorescent biosensors
that produce a luminescence ON response either on selective binding
or on reacting with an analyte that is produced through a specific
biochemical/enzymatic transformation. Studying such enzymatic processes
by probing the fluorescence response as the read-out signal is expected
to provide important insights into crucial biochemical transformations
induced by an enzyme in its native form. Many of such studies are
extended for monitoring enzymatic transformations under in vitro or
in vivo condition. A few of the recent reports reveal that such molecular
probes are even capable of quantifying abnormal levels of enzymes
in real-time and is linked to the key area of clinical diagnostics
and chemical biology. A synchronized analysis of all such reports
helps in developing a rationale for designing purpose-built molecular
probes or chemodosimeters as well as newer reagents for studying crucial
enzymatic process or quantification of the respective enzyme. In this
review, an attempt will be there to highlight several recent bioimaging
reagents and studies that have provided insights into crucial biochemical
or enzymatic transformations.