se9b00848_si_001.pdf (23.49 MB)
Single-Molecule Protein Detection in a Biofluid Using a Quantitative Nanopore Sensor
journal contribution
posted on 2019-08-21, 17:33 authored by Avinash
Kumar Thakur, Liviu MovileanuProtein
detection in complex biological fluids has wide-ranging
significance across proteomics and molecular medicine. Existing detectors
cannot readily distinguish between specific and nonspecific interactions
in a heterogeneous solution. Here, we show that this daunting shortcoming
can be overcome by using a protein bait-containing biological nanopore
in mammalian serum. The capture and release events of a protein analyte
by the tethered protein bait occur outside the nanopore and are accompanied
by uniform current openings. Conversely, nonspecific pore penetrations
by nontarget components of serum, which take place inside the nanopore,
are featured by irregular current blockades. As a result of this unique
peculiarity of the readout between specific protein captures and nonspecific
pore penetration events, our selective sensor can quantitatively sample
proteins at single-molecule precision in a manner distinctive from
those employed by prevailing methods. Because our sensor can be integrated
into nanofluidic devices and coupled with high-throughput technologies,
our approach will have a transformative impact in protein identification
and quantification in clinical isolates for disease prognostics and
diagnostics.
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protein bait-containinghigh-throughput technologiesnontarget componentsnanofluidic devicesnanoporeSingle-Molecule Protein DetectionQuantitative Nanopore Sensor Protein detectionrelease eventssingle-molecule precisionpore penetrationsdisease prognosticsprotein identificationprotein baitpore penetration eventsprotein analytetransformative impact
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