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Simple and Efficient Solution-Phase Synthesis of Oligonucleotides Using Extractive Work-Up

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journal contribution
posted on 2006-11-17, 00:00 authored by Martijn C. de Koning, Amar B. T. Ghisaidoobe, Howard I. Duynstee, Paul B. W. Ten Kortenaar, Dmitri V. Filippov, Gijs A. van der Marel
A solution-phase synthesis protocol amenable to scale-up was developed for the preparation of oligonucleotides employing phosphoramidite chemistry and DMTr/iBu/Bz-protected monomers. Isolation of intermediates was accomplished by means of extractions as the only purification tool. The potential of the method is demonstrated with the synthesis of a hexameric DNA fragment in high yield and purity.

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