op060133q_si_001.pdf (1.49 MB)
Simple and Efficient Solution-Phase Synthesis of Oligonucleotides Using Extractive Work-Up
journal contribution
posted on 2006-11-17, 00:00 authored by Martijn C. de Koning, Amar B. T. Ghisaidoobe, Howard I. Duynstee, Paul B. W. Ten Kortenaar, Dmitri V. Filippov, Gijs A. van der MarelA solution-phase synthesis protocol amenable to scale-up was
developed for the preparation of oligonucleotides employing
phosphoramidite chemistry and DMTr/iBu/Bz-protected monomers. Isolation of intermediates was accomplished by means
of extractions as the only purification tool. The potential of the
method is demonstrated with the synthesis of a hexameric DNA
fragment in high yield and purity.