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Shotgun Proteomic Analysis Unveils Survival and Detoxification Strategies by Caulobacter crescentus during Exposure to Uranium, Chromium, and Cadmium
journal contribution
posted on 2014-04-04, 00:00 authored by Mimi C. Yung, Jincai Ma, Michelle R. Salemi, Brett
S. Phinney, Grant R. Bowman, Yongqin JiaoThe
ubiquitous bacterium Caulobacter crescentus holds
promise to be used in bioremediation applications due to its
ability to mineralize U(VI) under aerobic conditions. Here, cell free
extracts of C. crescentus grown in the presence of
uranyl nitrate [U(VI)], potassium chromate [Cr(VI)], or cadmium sulfate
[Cd(II)] were used for label-free proteomic analysis. Proteins involved
in two-component signaling and amino acid metabolism were up-regulated
in response to all three metals, and proteins involved in aerobic
oxidative phosphorylation and chemotaxis were down-regulated under
these conditions. Clustering analysis of proteomic enrichment revealed
that the three metals also induce distinct patterns of up- or down-regulated
expression among different functional classes of proteins. Under U(VI)
exposure, a phytase enzyme and an ABC transporter were up-regulated.
Heat shock and outer membrane responses were found associated with
Cr(VI), while efflux pumps and oxidative stress proteins were up-regulated
with Cd(II). Experimental validations were performed on select proteins.
We found that a phytase plays a role in U(VI) and Cr(VI) resistance
and detoxification and that a Cd(II)-specific transporter confers
Cd(II) resistance. Interestingly, analysis of promoter regions in
genes associated with differentially expressed proteins suggests that
U(VI) exposure affects cell cycle progression.
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bacterium Caulobacter crescentusoxidative stress proteinsHeat shockoxidative phosphorylationcell cycle progressionpromoter regionsShotgun Proteomic Analysis Unveils Survivalphytase enzymebioremediation applicationsacid metabolismDetoxification Strategiesproteomic enrichmentcadmium sulfateuranyl nitrateClustering analysisCrCaulobacter crescentusmembrane responsesABC transporterefflux pumps
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