bc9b00664_si_001.pdf (647.55 kB)
Sequential Electrostatic Assembly of a Polymer Surfactant Corona Increases Activity of the Phosphotriesterase arPTE
journal contribution
posted on 2019-10-18, 19:45 authored by William
H. Zhang, Benjamin M. Carter, Graham J. Day, Norman Govan, Colin Jackson, Adam W. PerrimanWe
present a new methodology for the generation of discrete molecularly
dispersed enzyme–polymer–surfactant bioconjugates. Significantly,
we demonstrate that >3-fold increase in the catalytic efficiency
of
the diffusion-limited phosphotriesterase arPTE can be achieved through
sequential electrostatic addition of cationic and anionic polymer
surfactants, respectively. Here, the polymer surfactants assemble
on the surface of the enzyme via ion exchange to yield a compact corona.
The observed rate enhancement is consistent with a mechanism whereby
the polymer–surfactant corona gives rise to a decrease in the
dielectric constant in the vicinity of the active site of the enzyme,
accelerating the rate-determining product diffusion step. The facile
methodology has significant potential for increasing the efficiency
of enzymes and could therefore have a substantially positive impact
for industrial enzymology.