ac9b01788_si_002.zip (0.76 kB)
Separation of 13C- and 15N‑Isotopologues of Amino Acids with a Primary Amine without Mass Resolution by Means of O‑Phthalaldehyde Derivatization and Collision Induced Dissociation
dataset
posted on 2019-10-18, 15:40 authored by Jannick Kappelmann, Martin Beyß, Katharina Nöh, Stephan NoackComputational
and experimental advances of recent years have culminated
in establishing 13C-Metabolic Flux Analysis (13C-MFA) as a routine methodology to unravel the fluxome. As the acronym
suggests, 13C-MFA has relied on the relative abundance
of 13C-isotopes in metabolites for flux inference, most
commonly measured by mass spectrometry. In this manuscript we expand
the scope of labeling measurements to the case of simultaneous 13C- and 15N-labeling of amino acids. Analytically,
the separation of isotopologues of this metabolite class can only
be achieved at resolving power beyond 65,000. In this manuscript we
harvest an overlooked property of the collision induced dissociation
of amino acid adducts to discern 13C- and 15N- isotopologues of amino acids with a primary amine without separating
them in the m/z domain.