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Sensitive and Site-Selective Determination of Phosphorylated Peptides with a Ratiometric Photoelectrochemical Strategy
journal contribution
posted on 2019-11-19, 17:07 authored by Baihe Fu, Zhonghai ZhangCurrently, the sensitive detection of phosphopeptides
is greatly
significant and necessary in the fields of medicinal and bioanalytical
chemistry, and the main implementation is through a mass spectrometric
(MS) method. However, MS methods are very complicated and expensive
and are not capable of site-selective determination of phosphorylated
amino acid residues. The exploration of a facile and reliable technique
with high reproducibility that can both sensitively discriminate the
normal and phosphorylated peptides and, more importantly, distinguish
the different phosphorylated sites in peptides is highly desirable.
Herein, we propose a ratiometric photoelectrochemical (PEC) strategy
based on the totally opposite PEC response of phosphopeptides on rutile
and anatase TiO2-based photoelectrodes, which allows us
to sensitively distinguish the nonphosphopeptides and phosphopeptides,
to detect the concentrations of the phosphopeptides, and to recognize
the phosphorylated residues in a site-selective sensing model. The
ratiometric PEC strategy significantly minimizes the operation process,
simplifies instrumentation, and has promising and wide applications
in biology, analytical chemistry, and clinical diagnostics.
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Keywords
Phosphorylated Peptidesratiometric PEC strategyanatase TiO 2phosphorylated peptidesacid residuesSite-Selective DeterminationPEC responseoperation processphosphopeptideratiometric photoelectrochemicalphosphorylated sitesMS methodsphosphorylated residuesRatiometric Photoelectrochemical Strategysite-selective determinationbioanalytical chemistry
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