sc8b02672_si_001.pdf (362.87 kB)
Self-Sufficient Flow-Biocatalysis by Coimmobilization of Pyridoxal 5′-Phosphate and ω‑Transaminases onto Porous Carriers
journal contribution
posted on 2018-08-26, 00:00 authored by Ana I. Benítez-Mateos, Martina L. Contente, Susana Velasco-Lozano, Francesca Paradisi, Fernando López-GallegoWe
expanded the application of self-sufficient heterogeneous biocatalysts
containing coimmobilized ω-transaminases and pyridoxal 5′-phosphate
(PLP) to efficiently operate packed-bed reactors in continuous flow.
Using a ω-transaminase from Halomonas elongata coimmobilized with PLP onto porous methacrylate-based carriers coated
with polyethylenimine, we operated a packed-bed reactor continuously
for up to 50 column volumes at 1.45 mL × min–1 in the enantioselective deamination of model amines (α-methylbenzyl
amine), yielding >90% conversion in all cycles without exogenous
addition
of cofactor. In this work, we expanded the concept of self-sufficient
heterogeneous biocatalysts to other ω-transaminases such as
the ones from Chromobacterium violaceum and Pseudomonas fluorescens. We found that enzymes with lower
affinities toward PLP present lower operational stabilities in flow,
even when coimmobilizing PLP. Furthermore, ω-transaminases coimmobilized
with PLP were successfully implemented for the continuous synthesis
of amines and the sustainable metrics were assessed. These results
give some clues to exploit PLP-dependent ω-transaminases under
industrially relevant continuous operations in a more cost-effective
and environmentally friendly process.
History
Usage metrics
Categories
Keywords
exogenous additionPLP-dependent ω- transaminasescoimmobilizing PLPmethacrylate-based carriersChromobacterium violaceumpacked-bed reactorsbiocatalystcoimmobilized ω- transaminasesSelf-Sufficient Flow-Biocatalysisω- transaminases50 column volumesω- transaminaseenantioselective deaminationaminesPorous CarriersPseudomonas fluorescensHalomonas elongata coimmobilizedpacked-bed reactorω- transaminases coimmobilizedflow
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC