ab9b01446_si_001.pdf (408.63 kB)
Secretome-Based Prediction of Three-Dimensional Hepatic Microtissue Physiological Relevance
journal contribution
posted on 2019-12-08, 19:29 authored by Amish Asthana, Charles McRae White, Kenneth Ndyabawe, Megan Douglass, William S. KisaalitaEarly biomarkers for indication of the complex physiological
relevance
(CPR) of a three-dimensional (3D) tissue model are needed. CPR is
detected late in culture and requires different analytical techniques.
Albumin production, CYP3A4 expression, and formation of bile canaliculi
structures are commonly used to compare in vitro hepatic cells to
their in vivo counterpart. A universal biomarker independent of the
cell type would bring this to a common detection platform. We make
the case that these hepatic characteristics are not sufficient to
differentiate traditional (2D) cell culture from the more complex
3D culture. We explored the cytokine secretion profile (secretome)
for its potential as a 3D early culture biomarker. PDGF-AB/BB and
vascular endothelial growth factor (VEGF) were found to be upregulated
in 3D compared to 2D cultures at early time points (days 3 and 4).
These observations provide a foundation upon which in vivo validation
of cytokines can lead to physiologically relevant 3D in vitro cell
culture.