bi061467a_si_001.pdb (203.23 kB)
Roles of Tyr13 and Phe219 in the Unique Substrate Specificity of Pepsin B†
dataset
posted on 2006-12-05, 00:00 authored by Takashi KageyamaPepsin B is known to be distributed throughout mammalia, including carnivores. In this study,
the proteolytic specificity of canine pepsin B was clarified with 2 protein substrates and 37 synthetic
octapeptides and compared with that of human pepsin A. Pepsin B efficiently hydrolyzed gelatin but very
poorly hydrolized hemoglobin. It was active against only a group of octapeptides with Gly at P2, such as
KPAGF↓LRL and KPEGF↓LRL (arrows indicate cleavage sites). In contrast, pepsin A hydrolyzed
hemoglobin but not gelatin and showed high activity against various types of octapeptides, such as
KPAEF↓FRL and KPAEF↓LRL. The specificity of pepsin B is unique among pepsins, and thus, the enzyme
provides a suitable model for analyzing the structure and function of pepsins and related aspartic proteinases.
Because Tyr13 and Phe219 in/around the S2 subsites (Glu/Ala13 and Ser219 are common in most pepsins)
appeared to be involved in the specificity of pepsin B, site-directed mutagenesis was undertaken to replace
large aromatic residues with small residues and vice versa. The Tyr13Ala/Phe219Ser double mutant of
pepsin B was found to demonstrate broad activity against hemoglobin and various octapeptides, whereas
the reverse mutant of pepsin A had significantly decreased activity. According to molecular modeling of
pepsin B, Tyr13 OH narrows the substrate-binding space and a peptide with Gly at P2 might be preferentially
accommodated because of its high flexibility. The hydroxyl can also make a hydrogen bond with nitrogen
of a P3 residue and fix the substrate main chain to the active site, thus restricting the flexibility of the
main chain and strengthening preferential accommodation of Gly at P2. The phenyl moiety of Phe219 is
bulky and narrows the S2 substrate space, which also leads to a preference for Gly at P2, while lowering
the catalytic activity against other peptide types without making a hydrogen-bonding network in the active
site.