Ribose 2‘-F Labeling: A Simple Tool for the Characterization of RNA Secondary Structure Equilibria by 19F NMR Spectroscopy
2005-08-24T00:00:00Z (GMT) by
We present a facile 19F NMR spectroscopic approach for the characterization of bistable RNA. The concept is based on the site-specific labeling of RNA with single 2‘-deoxy-2‘-fluoro (2‘-F) nucleosides. Depending on the position of the 2‘-F label (either within a double helix or a single-stranded region), significantly different 19F chemical shifts were observed and assigned to the particular alternative folds. This has been exemplified for a series of bistable RNAs. The approach is promising for the detailed investigation of complex RNA folding pathways.
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