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Reversible Monolayer–Bilayer Transition in Supported Phospholipid LB Films under the Presence of Water: Morphological and Nanomechanical Behavior
journal contribution
posted on 2017-07-10, 00:00 authored by Silvia Ruiz-Rincón, Alejandro González-Orive, Jesús M. de la Fuente, Pilar CeaMixed
monolayer Langmuir–Blodgett (LB) films of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and cholesterol (Chol)
in the 1:1 ratio have been prepared onto solid mica substrates. Upon
immersion in water or in an aqueous HEPES solution (pH 7.4) the monolayer
LB films were spontaneously converted into well-organized bilayers
leaving free mica areas. The process has been demonstrated to be reversible
upon removal of the aqueous solution, resulting in remarkably free
of defects monolayers that are homogeneously distributed onto the
mica. In addition, the nanomechanical properties exhibited by the
as-formed bilayers have been determined by means of AFM breakthrough
force studies. The bilayers formed by immersion of the monolayer in
an aqueous media exhibit nanomechanical properties and stability under
compression analogous to those of DPPC:Chol supported bilayers obtained
by other methods previously described in the literature. Consequently,
the hydration of a monolayer LB film has been revealed as an easy
method to produce well-ordered bilayers that mimic the cell membrane
and that could be used as model cell membranes.
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HEPES solutionmica areasPhospholipid LB Filmsglycero -3-phosphocholineas-formed bilayersmonolayer LB filmmedia exhibit nanomechanical propertiesmonolayer LB filmsmica substratesmethodwell-ordered bilayersDPPCimmersioncell membranenanomechanical propertiesdefects monolayersAFM breakthrough force studiesmodel cell membranes
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