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Regulation of Interleukin‑6 in Head and Neck Squamous Cell Carcinoma Is Related to Papillomavirus Infection
journal contribution
posted on 2014-02-07, 00:00 authored by Ida Chiara Guerrera, Ivan Quetier, Rachid Fetouchi, Frederique Moreau, Christelle Vauloup-Fellous, Bouchra Lekbaby, Caroline Rousselot, Cerina Chhuon, Aleksander Edelman, Marine Lefevre, Jean-Claude Nicolas, Dina Kremsdorf, Jean Lacau Saint Guily, Patrick SoussanThe prevalence of head and neck squamous
cell carcinoma (HNSCC)
related to human papillomavirus (HPV) is increasing, unlike tobacco-
and alcohol-associated cancers. To gain a clearer understanding of
the molecular mechanisms implicated in HNSCC, depending on the presence
or not of a viral sequence, we investigated the expression of proteins
detected in the tumor regions of HNSCC patients. Twenty-two untreated
HNSCC patients were selected according to the presence of HPV-16.
For six patients, tumor and controlateral healthy tissues were tested
for viral detection before quantitative proteomic analysis. After
confirmation by Western blot, proteins were connected into a network,
leading to investigate interleukin-6 (IL-6) by immunocytochemistry
and ELISA. 41 ± 5% of proteins quantified by proteomics were
differentially expressed in tumor compared with healthy regions. Among
them, 36 proteins were retained as modulated in HPV-16 positive or
negative tumors, including cytokeratins, tubulins, annexin A1, and
serpin B1. Network analysis suggested a central role of IL-6, confirmed
by
overexpression of IL-6 in tumor tissues as in sera of HPV-negative
HNSCC compared with HPV-16-positive tumors. This modulation may contribute
to the survival and proliferation of cancer cells, although it was
not related to tumor stage or to the level of HPV-16 DNA.