bm8b00258_si_001.pdf (515.35 kB)
Recyclable Thermoresponsive Polymer−β-Glucosidase Conjugate with Intact Hydrolysis Activity
journal contribution
posted on 2018-04-18, 00:00 authored by Ishita Mukherjee, Sushant K. Sinha, Supratim Datta, Priyadarsi Deβ-Glucosidase
(BG) catalyzes the hydrolysis of cellobiose
to glucose and is a rate-limiting enzyme in the conversion of lignocellulosic
biomass to sugars toward biofuels. Since the cost of enzyme is a major
contributor to biofuel economics, we report the bioconjugation of
a temperature-responsive polymer with the highly active thermophilic
β-glucosidase (B8CYA8) from Halothermothrix orenii toward improving enzyme recyclability. The bioconjugate, with a
lower critical solution temperature (LCST) of 33 °C withstands
high temperatures up to 70 °C. Though the secondary structure
of the enzyme in the conjugate is slightly distorted with a higher
percentage of β-sheet like structure, the stability and specific
activity of B8CYA8 in the conjugate remains unaltered up to 30 °C
and retains more than 70% specific activity of the unmodified enzyme
at 70 °C. The conjugate can be reused for β-glucosidic
bond cleavage of cellobiose for at least four cycles without any significant
loss in specific activity.