Reconfigurable Surface with Photodefinable Physicochemical Properties for User-Designable Cell Scaffolds

Surfaces with specific topography and chemical composition are quite useful in many applications ranging from functional interfaces to cell incubation scaffolds. Although these surfaces can be easily fabricated by combining topography-construction methods and surface-functionalization strategies, their properties are often static after fabrication or merely switchable between “on” and “off” states. Developing surfaces that can be on-demand regulated are quite important for the generation of smart surfaces for future applications. In this paper, we present a reconfigurable surface with adjustable topography and chemical functionality utilizing the photodynamic feature of the disulfide bond. Structured surfaces, composed of disulfide-cross-linked polymer networks, were prepared by using disulfide-containing methacrylate as the monomer. We show that the topography and chemical functionality of the surface can be on-demand regulated after its fabrication, with 254 and 365 nm UV light, respectively, allowing to “define” the physicochemical properties of the surface using light before the usage. We also demonstrate the application of such surface as a user-designable cell scaffold, that different cell scaffolds can be generated from one original surface with a simple exposure process, to define the desired bioactivity onto every point of the surface and therefore exactly control cell behaviors on the scaffold.