ao7b01147_si_001.pdf (177.16 kB)
Rapid and Easy Extracellular Vesicle Detection on a Surface-Functionalized Power-Free Microchip toward Point-of-Care Diagnostics
journal contribution
posted on 2017-10-12, 16:18 authored by Ryo Ishihara, Tadaaki Nakajima, Yoshitaka Uchino, Asuka Katagiri, Kazuo Hosokawa, Mizuo Maeda, Yasuhiro Tomooka, Akihiko KikuchiExtracellular vesicles (EVs) are
promising novel cancer biomarkers.
However, rapid and easy analysis of EVs is challenging because conventional
detection methods require large sample volumes and long detection
times. Microchip-based analytical systems have particularly attracted
attention for development of point-of-care (POC) diagnostics. Previously,
various biomarker detection methods on a portable power-free poly(dimethylsiloxane)
(PDMS) microchip using laminar flow-assisted dendritic amplification
have been developed. Recently, for easy functionalization, we proposed
a microchannel inner surface-functionalized power-free PDMS microchip
(SF-PF microchip) utilizing electron beam-induced graft polymerization.
In this study, we apply the technique and prepare a novel SF-PF microchip.
On the microchip, EVs were successfully detected. The required sample
volume was 1.0 μL, and the total analysis time was 20 min. The
microchip can contribute to EV-based POC cancer diagnosis.
History
Usage metrics
Keywords
20 minlaminar flow-assisted dendritic amplificationanalysis timeEV-based POC cancer diagnosisnovel cancer biomarkersbiomarker detection methodsdetection timessample volumesurface-functionalized power-free PDMS microchipSurface-Functionalized Power-Free MicrochipPoint-of-Care Diagnostics Extracellular vesiclessample volumeselectron beam-induced graft polymerizationnovel SF-PF microchipEasy Extracellular Vesicle DetectionSF-PF microchip1.0 μ Ldetection methods
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC