ac9b04810_si_002.mp4 (2.77 MB)
Rapid Electrochemical Monitoring of Bacterial Respiration for Gram-Positive and Gram-Negative Microbes: Potential Application in Antimicrobial Susceptibility Testing
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posted on 2020-02-25, 17:08 authored by Pragadeeshwara Rao R, Shalini Sharma, Tithi Mehrotra, Rajashree Das, Ranjit Kumar, Rachana Singh, Indrajit Roy, Tinku BasuAntimicrobial resistance
is a grave threat to human life. Currently
used time-consuming antibiotic susceptibility test (AST) methods limit
physicians in selecting proper antibiotics. Hence, we developed a
rapid AST using electroanalysis with a 15 min assay time, called EAST,
which is live-monitored by time-lapse microscopy video. The present
work reports systematical electrochemical analysis and standardization
of protocol for EAST measurement. The proposed EAST is successfully
applied for Gram-positive Bacillus subtilis and Gram-negative Escherichia coli as model organisms to monitor bacterial
concentration, decay kinetics in the presence of various antibiotics
(ciprofloxacin, cefixime, and amoxycillin), drug efficacy, and IC50. Bacterial decay kinetics in the presence of antibiotics
were validated by the colony counting method, field emission scanning
electron microscopy, and atomic force microscopy image analysis. The
EAST predicts the antibiotic susceptibility of bacteria within 15
min, which is a significant advantage over existing techniques that
consume hours to days. The EAST was explored further by using bacteria-friendly l-lysine-functionalized cerium oxide nanoparticle coated indium
tin oxide as a working electrode to observe the enhanced electron-transfer
rate in the EAST. The results are very significant for future miniaturization
and automation. The proposed EAST has huge potential in the development
of a rapid AST device for applications in the clinical and pharmaceutical
industries.
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antibiotic susceptibility15 minAntimicrobial Susceptibility Testing Antimicrobial resistancefuture miniaturizationbacteria-friendly l-lysine-functionalized cerium oxide nanoparticleindium tin oxideGram-positive Bacillus subtilistime-lapse microscopy videofield emission scanning electron microscopyantibiotic susceptibility testGram-Negative MicrobesRapid Electrochemical Monitoring15 min assay timedecay kineticsGram-negative Escherichia coliEAST measurementelectron-transfer rateforce microscopy image analysisIC 50Bacterial Respirationmethods limit physiciansPotential Applicationmodel organismsBacterial decay kineticsAST devicework reports systematical electrochemical analysis
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