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Quantum-Dot-Based Lateral Flow Immunoassay for Detection of Neonicotinoid Residues in Tea Leaves
journal contribution
posted on 2017-10-27, 00:00 authored by Shuangjie Wang, Ying Liu, Shasha Jiao, Ying Zhao, Yirong Guo, Mengcen Wang, Guonian ZhuNeonicotinoid
insecticides are commonly used for pest control on
tea plantations as a result of their broad-spectrum activity. However,
neonicotinoid residues released from tea leaves into tea infusions
pose a dietary risk to consumers. Therefore, a rapid, sensitive, and
reliable on-site detection method for neonicotinoids is needed. We
developed a quantum-dot-based fluorescent lateral flow immunochromatographic
strip (LFICS) combined with a broad-specific antibody for detection
of typical neonicotinoids (imidacloprid, imidaclothiz, and clothianidin),
with sensitivities [50% inhibitory concentration (IC50)]
of 0.104–0.33 ng/mL and visual detection limits of 0.5–1
ng/mL. The strip assay could be completed in less than 30 min. Using
the LFICS to analyze spiked tea samples (green tea, black tea, and
oolong tea), the average recovery of the three neonicotinoids ranged
between 71 and 111%, with the coefficient of variation below 12%.
The results from the LFICS tests for field samples were consistent
with results from ultraperformance liquid chromatography–tandem
mass spectrometry. The newly developed strip is a useful tool for
the on-site detection of neonicotinoid residues in tea.