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Quantitative Proteomic Analysis of Chikungunya Virus-Infected Aedes aegypti Reveals Proteome Modulations Indicative of Persistent Infection
dataset
posted on 2020-05-21, 16:43 authored by Yingjun Cui, Pei Liu, Brian P. Mooney, Alexander W. E. FranzThe
mosquito-borne chikungunya virus (CHIKV) poses a threat to
human health in tropical countries throughout the world. The molecular
interactions of CHIKV with its mosquito vector Aedes aegypti are not fully understood. Following oral acquisition of CHIKV via
salinemeals, we analyzed changes in the proteome of Ae. aegypti in 12 h intervals by label-free quantification using a timsTOF Pro
mass spectrometer. For each of the seven time points, between 2647
and 3167 proteins were identified among CHIKV-infected and noninfected
mosquito samples, and fewer than 6% of those identified proteins were
affected by the virus. Functional enrichment analysis revealed that
the three pathways, Endocytosis, Oxidative phosphorylation, and Ribosome
biogenesis, were enriched during CHIKV infection. On the other hand,
three pathways of the cellular RNA machinery and five metabolism related
pathways were significantly attenuated in the CHIKV-infected samples.
Furthermore, proteins associated with cytoskeleton and vesicular transport,
as well as various serine-type endopeptidases and metallo-proteinases,
were modulated in the presence of CHIKV. Our study reveals biological
pathways and novel proteins interacting with CHIKV in the mosquito.
Overall, CHIKV infection caused minor changes to the mosquito proteome
demonstrating a high level of adaption between the vector and the
virus, essentially coexisting in a nonpathogenic relationship. The
mass spectrometry data have been deposited to the MassIVE repository
(https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=abfd14f7015243c69854731998d55df1) with the data set identifier MSV000085115.
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CHIKV infectionmass spectrometry datatimsTOF Pro mass spectrometerChikungunya Virus-Infected Aedes aegyptiRNAProteome Modulations IndicativeMSVQuantitative Proteomic Analysisproteinnoninfected mosquito samplesmosquito-borne chikungunya viruspathway12 h intervalsFunctional enrichment analysismosquito vector Aedes aegypti
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