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Quantitative Interrelation between Atractylenolide I, II, and III in Atractylodes japonica Koidzumi Rhizomes, and Evaluation of Their Oxidative Transformation Using a Biomimetic Kinetic Model
journal contribution
posted on 2018-11-05, 11:50 authored by Jung-Hoon Kim, Yuvin Lee, Guemsan Lee, Eui-Jeong Doh, Seungwoo HongAnalytical methods based on ultraperformance
liquid chromatography/ion-trap mass spectrometry (UPLC/ion-trap MS)
were developed for quantification of atractylenolide I, II, and III
in the methanol extract of Atractylodes japonica rhizomes with a C18 column in an acidified water/acetonitrile
gradient eluent in an LC system, and ion-trap MS coupled with electrospray
ionization was employed under positive-ion mode. The three atractylenolides
were quantified in all A. japonica samples,
and the content of atractylenolide I, II, and III showed a significant
correlation to each other. Such high correlation was explained by
the mechanistic insights into the biosynthetic pathway of atractylenoide
III and I from atractylenoide II by using the biomimetic cytochrome
P450 model, [Fe(tmp)](CF3SO3) (tmp = meso-tetramesitylporphyrin). Atractylenolides could be transformed
by oxidation via the oxidative enzyme in the A. japonica plant. The present study first reports the first oxidative transformation
of atractylenolides using the heme iron model complex.
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AtractylenolideOxidative Transformationjaponica plantUPLCLC systemBiomimetic Kinetic Model Analytical methodsatractylenolideoxidative enzymepositive-ion modeatractylenoide IIbiomimetic cytochrome P 450 modelatractylenoide IIIelectrospray ionizationbiosynthetic pathwayjaponica samplesAtractylodes japonica rhizomesheme iron modeloxidative transformationAtractylodes japonica Koidzumi RhizomesC 18 columnQuantitative Interrelationion-trap MScorrelation
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