ac701958z_si_002.pdf (73.3 kB)
Quantitative Comparison between Microfluidic and Microtiter Plate Formats for Cell-Based Assays
journal contribution
posted on 2008-01-01, 00:00 authored by Huabing Yin, Nicola Pattrick, Xunli Zhang, Norbert Klauke, Hayley C. Cordingley, Steven J. Haswell, Jonathan M. CooperIn this paper, we compare a quantitative cell-based assay
measuring the intracellular Ca2+ response to the agonist
uridine 5‘-triphosphate in Chinese hamster ovary cells,
in both microfluidic and microtiter formats. The study
demonstrates that, under appropriate hydrodynamic conditions, there is an excellent agreement between traditional well-plate assays and those obtained on-chip for
both suspended immobilized cells and cultured adherent
cells. We also demonstrate that the on-chip assay, using
adherent cells, provides the possibility of faster screening
protocols with the potential for resolving subcelluar
information about local Ca2+ flux.