pr3005327_si_001.pdf (217.47 kB)
QconCAT Standard for Calibration of Ion Mobility-Mass Spectrometry Systems
journal contribution
posted on 2016-02-20, 07:35 authored by Ross Chawner, Bryan McCullough, Kevin Giles, Perdita
E. Barran, Simon J. Gaskell, Claire E. EyersIon mobility-mass spectrometry (IM-MS) is a useful technique
for
determining information about analyte ion conformation in addition
to mass/charge ratio. The physical principles that govern the mobility
of an ion through a gas in the presence of a uniform electric field
are well understood, enabling rotationally averaged collision cross
sections (Ω) to be directly calculated from measured drift times
under well-defined experimental conditions. However, such “first
principle” calculations are not straightforward for Traveling
Wave (T-Wave) mobility separations due to the range of factors that
influence ion motion through the mobility cell. If collision cross
section information is required from T-Wave mobility separations,
then calibration of the instruments using known standards is essential
for each set of experimental conditions. To facilitate such calibration,
we have designed and generated an artificial protein based on the
QconCAT technology, QCAL-IM, which upon proteolysis can be used as
a universal ion mobility calibration standard. This single unique
standard enables empirical calculation of peptide ion collision cross
sections from the drift time on a T-Wave mobility instrument.