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Proteomic Analysis of Peripheral Blood Mononuclear Cells after a High-Fat, High-Carbohydrate Meal with Orange Juice
journal contribution
posted on 2017-09-19, 00:00 authored by Daniela F. S. Chaves, Paulo C. Carvalho, Elisa Brasili, Marcelo M. Rogero, Neuza A. Hassimotto, Jolene K. Diedrich, James J. Moresco, John R. Yates, Franco M. LajoloOxidative stress and inflammation
play a role in the physiopathology of insulin resistance, diabetes
and cardiovascular disease. A single high-fat, high-carbohydrate (HFHC)
meal induces an increase in inflammatory and oxidative stress markers
in peripheral blood mononuclear cells (PBMC). Previous studies have
shown that orange juice is able to prevent this response by inhibiting
toll like receptors (TLR) expression and endotoxemia. Our goal was
to study the proteome response in PBMC after the consumption of a
HFHC meal consumed with water, orange juice or an isocaloric beverage
(water with glucose). Twelve healthy individuals completed the protocol
in a crossover design, and blood samples were obtained before and
1, 3, and 5 h after consumption. Proteomic profile, glucose, insulin,
lipid and cytokines levels were investigated. The glycemic and insulinemic
response was higher when the meal was consumed with glucose, while
there was no difference in the response between water and orange juice.
Proteome analysis in PBMC was carried out using TMT ten-plex. A total
of 3813 proteins, originating from 15 662 peptides were identified.
Three proteins showed significantly altered expression in the three
treatments: apolipoprotein A-II, ceruloplasmin and hemopexin. When
the HFHC meal was consumed with water there was an increase in some
inflammatory pathways such as the Fc-gamma receptor dependent phagocytosis
and the complement cascade, but the immune system as a whole was not
significantly altered. However, when the meal was consumed with glucose,
the immune system was up regulated. Among the pathways induced after
3 h were those of the adaptive immune system and cytokine signaling.
Five hours after the meal, pathways of the complement cascade and
classical antibody mediated complement activation were up regulated.
When the meal was consumed with orange juice there was an up regulation
of proteins involved in signal transduction, DNA replication and cell
cycle. The promyelocytic leukemia protein (PML) showed a 28.2-fold
increase. This protein was down regulated when the meal was consumed
with water. Regarding the immune system, several of the pathways induced
by glucose were down regulated when the meal was consumed with orange
juice: proteins involved with the adaptive immune system and cytokine
signaling. Therefore, we have shown that orange juice can not only
suppress diet induced inflammation, but also regulate the expression
of proteins such as PML, which may play a key role in the regulation
of metabolism.