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Proteome Analysis of Cold Response in Spring and Winter Wheat (Triticum aestivum) Crowns Reveals Similarities in Stress Adaptation and Differences in Regulatory Processes between the Growth Habits
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posted on 2013-11-01, 00:00 authored by Klára Kosová, Pavel Vítámvás, Sébastien Planchon, Jenny Renaut, Radomíra Vanková, Ilja Tom PrášilA proteomic
response to cold treatment (4 °C) has been studied
in crowns of a frost-tolerant winter wheat cultivar Samanta and a
frost-sensitive spring wheat cultivar Sandra after short-term (3 days)
and long-term (21 days) cold treatments. Densitometric analysis of
2-D differential in gel electrophoresis (2D-DIGE) gels has resulted
in the detection of 386 differentially abundant protein spots, which
reveal at least a two-fold change between experimental variants. Of
these, 58 representative protein spots have been selected for MALDI-TOF/TOF
identification, and 36 proteins have been identified. The identified
proteins with an increased relative abundance upon cold in both growth
habits include proteins involved in carbohydrate catabolism (glycolysis
enzymes), redox metabolism (thioredoxin-dependent peroxidase), chaperones,
as well as defense-related proteins (protein revealing similarity
to thaumatin). Proteins exhibiting a cold-induced increase in the
winter cultivar include proteins involved in regulation of stress
response and development (germin E, lectin VER2), while proteins showing
a cold-induced increase in the spring cultivar include proteins involved
in restoration of cell division and plant growth (eIF5A2, glycine-rich
RNA-binding protein, adenine phosphoribosyltransferase). These results
provide new insights into cold acclimation in spring and winter wheat
at the proteome level and enrich our previous work aimed at phytohormone
dynamics in the same plant material.
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Proteome Analysis5Aadenine phosphoribosyltransferasestress response36 proteinsCold Response58 representative protein spotswinter wheatgermin Eprotein spotsGrowth HabitsA proteomic responsewinter cultivarspring cultivarDensitometric analysiscell divisionplant growthStress AdaptationVER386 differentiallycarbohydrate catabolismproteome levelRegulatory Processesplant materialWinter WheatTriticum aestivumphytohormone dynamicsgrowth habits
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