mp9b01022_si_001.pdf (1.9 MB)
Protein–Sugar-Glass Nanoparticle Platform for the Development of Sustained-Release Protein Depots by Overcoming Protein Delivery Challenges
journal contribution
posted on 2019-12-03, 17:43 authored by Poulomi Polley, Shivam Gupta, Ruby Singh, Arpan Pradhan, Suparna Mercy Basu, Remya V., Sunil Kumar Yadava, Jyotsnendu GiriTherapeutic protein depots have limited clinical success
because
of the presence of critical preparation barriers such as low encapsulation,
uncontrolled release, and activity loss during processing and storage.
In the present study, we used our novel protein-nanoencapsulation
(into sugar-glass nanoparticle; SGnP) platform to prepare a protein
depot to overcome the abovementioned formidable challenges. The SGnP-mediated
microparticle protein depot has been validated using four model proteins
(bovine serum albumin, horseradish peroxidase, fibroblastic growth
factor, and epidermal growth factor) and model biodegradable poly(lactic-co-glycolic acid) polymer system. The results show that
our protein-nanoencapsulation-mediated platform provides a new generic
platform to prepare a protein depot through the conventional emulsion
method of any polymer and single/multiple protein systems. This protein
depot has the required pharmaceutical properties such as high encapsulation
efficiency, burst-free sustained release, and protein preservation
during processing and storage, making it suitable for off-the-shelf
use in therapeutic protein delivery and tissue engineering applications.