np2009412_si_001.pdf (1.93 MB)
Profiling of Alkaloids and Eremophilanes in Miracle Tea (Packera candidissima and P. bellidifolia) Products
journal contribution
posted on 2012-05-25, 00:00 authored by Mabel Fragoso-Serrano, Gabriela Figueroa-González, Erick Castro-Carranza, Francisco Hernández-Solis, Edelmira Linares, Robert Bye, Rogelio Pereda-MirandaCommercial preparations of the Mexican herbal drug known
as “miracle
tea” (Packera candidissima and P.
bellidifolia) have been profiled qualitatively by HPLC and
GC-MS. Eremophilanes (3–7) were the
major components found in
the hexane-soluble fraction, while pyrrolizidine alkaloids (PAs) were
identified in the alkaloid extracts. The content of free PAs and their
N-oxides was determined for a total of 22 samples, and the results
showed that the amount of these hepatotoxic compounds (0.0005–0.94%
free PAs; 0.0004–0.55% N-oxides), through the presence of retrorsine
(1) and senesionine (2) as the main constituents,
may reach toxic levels. Hexane-soluble extracts from commercial presentations
(dried whole plants) of both species afforded neoadenostylone (3), 6-(2-methylbutanoyloxy)-9-oxo-1-(10)-furanoeremophilene
(4), and epineoadenostylone (5), in addition
to methyl-4-hydroxyphenylacetate (8) and methyl-2-(1-hydroxy-4-oxocyclohexyl)acetate
(9). Also, epicacalone (6) and the new compound
2β-hydroxyneoadenostylone (7) were isolated from P. bellidifolia.