jf9b02821_si_001.pdf (429.61 kB)
Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis
journal contribution
posted on 2019-08-15, 18:06 authored by Ana Cristina Abreu, Alejandro Molina-Miras, Luis M. Aguilera-Sáez, Lorenzo López-Rosales, María del Carmen Cerón-García, Asterio Sánchez-Mirón, Lucía Olmo-García, Alegría Carrasco-Pancorbo, Francisco García-Camacho, Emilio Molina-Grima, Ignacio FernándezThis study assessed
the feasibility of an NMR metabolomics approach
coupled to multivariate data analysis to monitor the naturally present
or stresses-elicited metabolites from a long-term (>170 days) culture
of the dinoflagellate marine microalgae Amphidinium
carterae grown in a fiberglass paddlewheel-driven
raceway photobioreactor. Metabolic contents, in particular, in two
members of the amphidinol family, amphidinol A and its 7-sulfate derivative
amphidinol B (referred as APDs), and other compounds of interest (fatty
acids, carotenoids, oxylipins, etc.) were evaluated by altering concentration
levels of the f/2 medium nutrients and daily mean irradiance. Operating
with a 24 h sinusoidal light cycle allowed a 3-fold increase in APD
production, which was also detected by an increase in hemolytic activity
of the methanolic extract of A. carterae biomass. The presence of APDs was consistent with the antitumoral
activity measured in the methanolic extracts of the biomass. Increased
daily irradiance was accompanied by a general decrease in pigments
and an increase in SFAs (saturated fatty acids), MUFAs (monounsaturated
fatty acids), and DHA (docosahexaenoic acid), while increased nutrient
availability lead to an increase in sugar, amino acid, and PUFA ω-3
contents and pigments and a decrease in SFAs and MUFAs. NMR-based
metabolomics is shown to be a fast and suitable method to accompany
the production of APD and bioactive compounds without the need of
tedious isolation methods and bioassays. The two APD compounds were
chemically identified by spectroscopic NMR and spectrometric ESI-IT
MS (electrospray ionization ion trap mass spectrometry) and ESI-TOF
MS (ESI time-of-flight mass spectrometry) methods.
History
Usage metrics
Categories
Keywords
irradianceESI-IT MScarterae biomassAPD compoundsMarine Microalga Amphidinium carterae Unraveledpigmentstresses-elicited metabolitesconcentration levelslight cycledinoflagellate marine microalgae Amphidinium carteraeacidamphidinol familyPUFA ω-3 contentsESI time-of-flight mass spectrometryOther BioproductsSFAantitumoral activityisolation methodsmethanolic extractsspectroscopic NMR7- sulfateESI-TOF MSMUFAmultivariate data analysisNMR metabolomics approachelectrospray ionization ion trap mass spectrometryfiberglass paddlewheel-driven raceway photobioreactorbioactive compounds24 hNMR-based metabolomicsDHAamphidinol BResonance Metabolomics ApproachAPD productionMultivariate Data AnalysisMetabolic contents
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC